Guanajuato offers nonstop service to 23 destinations, served by 5 airlines.
| Destination | Code | Country | Distance | Duration | Airlines |
|---|---|---|---|---|---|
| Mexico City | NLU | Mexico | 291 km | 0h 55m | VB |
| Mexico City | MEX | Mexico | 305 km | 1h 15m | AM |
| Puebla | PBC | Mexico | 385 km | 1h 9m | Y4 |
| Puerto Vallarta | PVR | Mexico | 393 km | 1h 3m | Y4 |
| Monterrey | MTY | Mexico | 547 km | 1h 21m | VB, Y4 |
| San Jose Cabo | SJD | Mexico | 883 km | 1h 46m | Y4 |
| San Antonio | SAT | USA | 992 km | 1h 48m | VB |
| Houston | IAH | USA | 1,171 km | 2h 9m | UA, VB |
| Merida | MID | Mexico | 1,228 km | 2h 8m | VB |
| Ciudad Juarez | CJS | Mexico | 1,278 km | 2h 10m | VB |
| Dallas | DFW | USA | 1,390 km | 2h 30m | AA, VB |
| Cancun | CUN | Mexico | 1,517 km | 2h 29m | VB, Y4 |
| Mexicali | MXL | Mexico | 1,876 km | 2h 56m | Y4 |
| Tijuana | TIJ | Mexico | 1,998 km | 3h 8m | VB, Y4 |
| Ontario | ONT | USA | 2,146 km | 3h 17m | Y4 |
| Atlanta | ATL | USA | 2,186 km | 3h 30m | AM |
| Los Angeles | LAX | USA | 2,198 km | 3h 32m | Y4 |
| Fresno | FAT | USA | 2,485 km | 3h 45m | Y4 |
| Chicago | MDW | USA | 2,642 km | 3h 50m | Y4 |
| Chicago | ORD | USA | 2,652 km | 3h 55m | Y4 |
| San Jose | SJC | USA | 2,680 km | 3h 56m | Y4 |
| Oakland | OAK | USA | 2,723 km | 4h 13m | Y4 |
| Sacramento | SMF | USA | 2,749 km | 4h 13m | Y4 |